An active immunization approach to generate protective catalytic antibodies

An active immunization approach to generate protective catalytic antibodies.

We report that mice immunized with a phosphate immunogen produced polyclonal catalytic antibodies (PCAbs) that catalysed the hydrolysis of carbaryl, a widely used broad-spectrum carbamate insecticide that exerts toxic effects in animals and humans. The reaction catalysed by the PCAbs (IgGs) obeyed Michaelis-Menten kinetics in vitro with the following values at pH 8.0 and 25 degrees C: K(m) appr...

A new Approach to active Immunization of Sheep by a combined Sheep pox and Anthrax Vaccine

A cofactor approach to copper-dependent catalytic antibodies.

A strategy for the preparation of semisynthetic copper(II)-based catalytic metalloproteins is described in which a metal-binding bis-imidazole cofactor is incorporated into the combining site of the aldolase antibody 38C2. Antibody 38C2 features a large hydrophobic-combining site pocket with a highly nucleophilic lysine residue, Lys(H93), that can be covalently modified. A comparison of several...

EASIER: An Approach to Automatically Generate Active Ontologies for Intelligent Assistants

Intelligent assistants are ubiquitous and will grow in importance. Apple’s well-known assistant Siri uses Active Ontologies to process user input and to model the provided functionalities. Supporting new features requires extending the ontologies or even building new ones. The question is no longer “How to build an intelligent assistant?” but “How to do it efficiently?” We propose EASIER, an ap...

non-corrective approach to pronunciation

the aim of this study has been to find answers for the following questions: 1. what is the effect of immediate correction on students pronunciation errors? 2. what would be the effect of teaching the more rgular patterns of english pronunciation? 3. is there any significant difference between the two methods of dealing with pronuciation errore, i. e., correction and the teaching of the regular ...